Haemacytometer

Title
* Haemacytometer

Objective
* To perform manual cell count

Apparatus & angstrom; Materials
* Heamacytometer
* Blood sample (female)
* Distilled water
* Thoma RBC pipette
* Microscope
* Microscope slide
* Tally counter

Procedure
* Blood is wasted to the 0.5 mark on Thoma RBC pipette
* Then the diluting liquid to is drawn to the 101 mark
* The mixture is then shake for 5 minutes
* The cover glass is placed over the hemacytometer chamber.
* With a Pasteur or transfer pipette, both chambers of the hemacytometer are change (without overflow) by capillary action. Cells will settle in the furnish and in the pipet by gravity within a few seconds.
* Worked quickly.
* Using the microscope with a 10X ocular (and a 10X objective), the cells in each of 10 squares (1 mm2 each) are counted. If over 10% of the cells understand clumps, paraphrase entire sequence. If fewer than 200 or more than than 500 cells are present in the 10 squares, repeat with a more suitable dilution factor.
* The number of cells per ml is calculated, and the intact number of cells, in the original culture as follows: Cells/ml = average count per square x 104 Total cells = cells per ml X any dilution factor X total pot of cell preparation from which the sample was taken.
* Counting repeated to fleck reproducibility (+/- 15%).





Result
Square| Cell counted|
A| 90|
B| 88|
C| 81|
D| 98|
E| 79|
Total| 436|

* Calculation
= Avg ×DFA mm2×D (0.1 mm )

= 436 ×2000.20 mm2× (0.1 mm )

= 436 ×10,000/?L

=4,360,000 (or 4.36 ×106)/?L or 4.36 × 1012/L

Discussion
* Normal background
Adult Male| 4.5-6.0 × 1012 / L|
Adult Female| 4.0-5.5 × 1012 / L|
Newborn| 5.0-6.3 × 1012 / L|

* Sources of error
* Falsely high counts
* Collection of line of business from the area where there is hemoconcentration.
* Inadequate wiping of the pipette.
* Improper mixing.
* Uneven distribution in...If you want to get a full essay, order it on our website: Ordercustompaper.com



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